Test Method Development and Diagnostic Tools for chemical effects

images showing development of diagnostic tools: fathead minnow and population modelling

We have developed in vivo testing systems for endocrine active chemicals using the fathead minnow and zebrafish and the data from this research have been used in the development of International OECD and US EPA test guidelines for hazard identification. These test systems have included early life stage tests and reproductive performance assays. Some of the assays developed in our laboratory for vitellogenin have been commercialised and used in research and industry laboratories worldwide. We have applied the screening and testing methods developed for endocrine active chemicals to complex mixtures. In collaboration with the University of Birmingham Dr Mark Viant) we are investigating the use of metabolomics in diagnosing for endocrine disrupting effects, both in the laboratory under controlled conditions and in wild fish populations. With a strong desire to reduce animal use in our research, the EABRG employs a wide range of in vitro techniques, including recombinant yeasts, fish cell lines and primary fish cell cultures to screen for chemical effects and we are involved with a series of projects developing population models to predict for chemical effects in wildlife populations.

Ecotoxicology and Aquatic Biology Research Group

Test Method Development and Diagnostic Tools for chemical effects